We compared the performance of three commercial Hiv-1 /HIV-2 combination EIAs in detecting antibodies to HIV-2; Genetic systems(kit-I), Behring(kit-II) and Korean Green Cross(kit-III). We tested in 506 serum samples, including 15 anti-HIV-1
positive
samples and 11 anti-HIV-2 positive samples. During EIV procedure, the kit-I was very inconvenient because we-had to dilute the serum in the tube and then transfer it to well one by one. In the kit-II, the prewashing steps were troublesome.
The results were as follows.
1. In positive samples, the kit-II and the kit-III showed 100% sensitivity, but the kit-I was less sensitive(84.6%).
2. In seronegative samples, the frequencies of false positive rates were 1.46%(kit-I), 1.04% (kit-II) and 0.83% (kit-III), respectively.
3. The kit-I was significantly less sensitive(P=0.016) than other two kits, but all three kits showed similar specificity.
4. There were no statistically significant differences in diagnostic efficacy between three kits when they were compared by relative operating characteristic curves.
Testing for both anti-HIV-1 and HIV-2 in all persons undergoing testing for HIV infection is not currently desirable. However, it should be considered that substitute a Hiv-1/HIV-2 combination EIA for the HIV-1 EIA currently used to screen blood
donors
because increased number of HIV infection and serious effects of AIDS to public health.
Continued case and serologic surveillance will be needed to monitor the epidemiology of HIV-2 infection in Korea.
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